[HTML][HTML] Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3
KR Karlsson, S Cowley, FO Martinez, M Shaw… - Experimental …, 2008 - Elsevier
OBJECTIVE: To develop a simple and efficient method for producing homogeneous
populations of monocytes and macrophages from human embryonic stem cells (hES).
MATERIALS AND METHODS: Human embryonic stem cell lines KCL001, KCL002, and
HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth
factors using a three-step method. The method involved embryoid body (EB) formation in
hES media, directed differentiation with macrophage colony-stimulating factor and …
populations of monocytes and macrophages from human embryonic stem cells (hES).
MATERIALS AND METHODS: Human embryonic stem cell lines KCL001, KCL002, and
HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth
factors using a three-step method. The method involved embryoid body (EB) formation in
hES media, directed differentiation with macrophage colony-stimulating factor and …
