The complexity of the lymphostromal interplay that is essential to αβT-cell development is
reflected by the heterogeneity of both lymphocytes and thymic stromal cells. While panels of
monoclonal antibodies have described many of the cellular components of these
microenvironments, the means to quantify stromal cell subsets using flow cytometry remains
poorly defined. This study refines and compares various stromal cell isolation procedures
and determines the effects of various digestion enzymes on important surface molecules …