[HTML][HTML] Purification of P-TEFb, a transcription factor required for the transition into productive elongation (∗)
NF Marshall, DH Price - Journal of Biological Chemistry, 1995 - ASBMB
Production of full-length runoff transcripts in vitro and functional mRNA in vivo is sensitive to
the drug 5, 6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). We previously proposed
the existence of an activity, P-TEF (positive transcription elongation factor) that functions in a
DRB-sensitive manner to allow RNA polymerase II elongation complexes to efficiently
synthesize long transcripts (Marshall, NF and Price, DH (1992) Mol. Cell. Biol. 12, 2078-
2090). We have fractionated nuclear extracts of Drosophila melanogaster K c cells and …
the drug 5, 6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). We previously proposed
the existence of an activity, P-TEF (positive transcription elongation factor) that functions in a
DRB-sensitive manner to allow RNA polymerase II elongation complexes to efficiently
synthesize long transcripts (Marshall, NF and Price, DH (1992) Mol. Cell. Biol. 12, 2078-
2090). We have fractionated nuclear extracts of Drosophila melanogaster K c cells and …