Inducing an anergic state in mast cells and basophils without secretion
D MacGlashan Jr, BJ Undem - Journal of allergy and clinical immunology, 2008 - Elsevier
BACKGROUND: IgE-mediated secretion from mast cells or basophils depends on the
activity of both spleen tyrosine kinase (syk) and phosphatidyl inositol 3′ kinase (PI3K), but
several specific downregulatory pathways (eg, loss of syk expression) do not. OBJECTIVE:
We tested whether stimulation with antigen in the presence of a syk inhibitor (NVP-QAB205)
would ablate secretion while simultaneously allowing anergy. METHODS: The anergic or
desensitized state in human basophils, cultured-derived mast cells, and in situ stimulated …
activity of both spleen tyrosine kinase (syk) and phosphatidyl inositol 3′ kinase (PI3K), but
several specific downregulatory pathways (eg, loss of syk expression) do not. OBJECTIVE:
We tested whether stimulation with antigen in the presence of a syk inhibitor (NVP-QAB205)
would ablate secretion while simultaneously allowing anergy. METHODS: The anergic or
desensitized state in human basophils, cultured-derived mast cells, and in situ stimulated …
